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(R1057) Quick-RNA Miniprep Plus Kit (50 prep)

HIGHLIGHTS

  • Broad Range: Extract total RNA (including small/micro RNAs) from any sample.
  • DNA-Free: Genomic DNA removal column and DNase I included.
  • NGS-Ready: RNA is ready for all downstream applications including Next-Gen Sequencing, RT-qPCR, hybridization, etc.
Protocol: 
Data Sheet: 

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Descripción

DESCRIPTION

The Quick-RNA Miniprep Plus Kit is one of the most innovative RNA isolation kits available, designed for the easy, reliable, and rapid isolation of DNA-free RNA from cells, all tissue types, whole blood, and biological fluids. The provided DNA/RNA Shield stabilizes samples, allowing them to be stored without the need for immediate freezing or processing for up to one month. Furthermore, DNA/RNA Shield™ inactivates RNases as well as microbial pathogens (viruses, bacteria, etc.). The procedure combines a unique buffer system with Zymo-Spin Column technology to yield high quality total RNA (including small RNAs 17-200 nt). Simply add DNA/RNA Shield and Proteinase K to extract total RNA from any tissue, then purify the RNA using the Zymo-Spin Column. The result is highly-concentrated, DNA-free RNA that is suitable for RT-PCR, hybridization, sequencing, etc. In addition, the kit can be used for the enrichment of small and large RNAs in two separate fractions.

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TECHNICAL SPECIFICATIONS

Equipment Microcentrifuge, vortex, heat block/bath (55ºC)
Purity RNA is ready for Next-Gen sequencing, RTPCR, microarray, hybridization, etc. A260/A280, A260/A230: >1.8.
Sample Source Any cells (animal, blood cells etc.), all tissues (tough-to-lyse, FFPE, etc.), blood, biological fluids, and enzymatic reactions (e.g., DNase I), samples in DNA/RNA Shield™, TRIzol® or RNAlater™.
Size Range Total RNA ≥ 17 nt
Yield spin column, 100 µg RNA (binding capacity), ≥50 µl (elution volume)
Supplemental Info

Información adicional

Peso 0,0 kg
Dimensiones 0,0 × 0,0 × 0,0 cm