Descripción
DESCRIPTION
The RNA Clean & Concentrator-5 kits are RNA clean up kits that provide a simple and reliable method for the rapid preparation of high-quality, RT-PCR-ready, DNA-free (R1013, R1014) RNA. This simple procedure is based on the use of a unique single-buffer system and Zymo-Spin column technology that allows for selective recovery of total RNA (> 17 nt), large RNAs (> 200 nt), and/or small RNAs (17-200 nt). The procedure is easy: Add binding buffer and ethanol to your sample, then bind, wash and elute ultra pure RNA. The RNA can be eluted from the Zymo-Spin IC Column in as little as ≥ 6 µl of RNase-free water. The highly-concentrated, purified RNA is suitable for all subsequent analyses and molecular manipulations.
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Equipment |
Microcentrifuge |
Format |
Spin-Column |
Purity |
RNA is ready for Next-Gen sequencing, RT-PCR, microarray, hybridization, etc. A260/A280, A260/A230: > 1.8. |
Sample Source |
DNase I treated RNA, in vitro transcription products, the aqueous phase following TRIzol/chloroform or similar extraction |
Size Range |
Total RNA ≥ 17 nt |
Yield |
10 µg RNA (binding capacity), ≥ 6 µl (elution volume) |
Supplemental Info |
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