Descripción
DESCRIPTION
The Quick-DNA Fungal/Bacterial Midiprep Kit is designed for the simple and rapid isolation of DNA from tough-to-lyse fungi, including A. fumigatus, C. albicans, N. crassa, S. cerevisiae, S. pombe, as well as Gram (+/-) bacteria, algae, and protozoa. The procedure is easy and can be completed in minutes: fungal and/or bacterial samples are rapidly and efficiently lysed with our state of the art, ultra-high density BashingBeads. Zymo-Spin column technology is then used to isolate the DNA that is ideal for downstream molecular-based applications including PCR, array, etc.
Applicable For |
All sensitive downstream applications such as qPCR and Next-Generation Sequencing. |
Elution Volume |
≥ 150 µl |
Equipment |
Centrifuge, Vacuum Source and Manifold, Microcentrifuge, Cell Disrupter/Pulverizer w/ 50 ml Tube Adapter |
Processing Time |
≤ 20 minutes |
Processing Volume |
≤ 500mg fungi or bacteria (wet weight), 5×109 bacterial cells, 5×108 yeast cells, or 5×107 mammalian cells |
Purity |
Typical A260/A280 & A260/A230 ≥ 1.8 |
Sample Source |
Fungal and bacterial cell cultures, spores, pollen, nematodes, as well as other microorganisms can also be sampled. |
Size Range |
Capable of recovering genomic DNA up to and above ≥40 kb. Typical fragment sizes range from 25 to 35 kb. If present, parasitic and viral DNA will also be recovered. |
Type |
Total DNA |
Yield |
≤ 125 µg total DNA |
Supplemental Info |
|