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(D6105) Quick-DNA Fungal/Bacterial Midiprep Kit (25 preps)

HIGHLIGHTS

  • Boost Detection: Included BashingBeads ensure complete lysis of tough-to-lyse samples.
  • Ultra-Pure: Ready for qPCR, Next-Gen Sequencing, arrays, etc.
  • Simple: Fastest workflow (≤ 20 minutes).
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Descripción

DESCRIPTION

The Quick-DNA Fungal/Bacterial Midiprep Kit is designed for the simple and rapid isolation of DNA from tough-to-lyse fungi, including A. fumigatusC. albicansN. crassaS. cerevisiae, S. pombe, as well as Gram (+/-) bacteria, algae, and protozoa. The procedure is easy and can be completed in minutes: fungal and/or bacterial samples are rapidly and efficiently lysed with our state of the art, ultra-high density BashingBeads. Zymo-Spin column technology is then used to isolate the DNA that is ideal for downstream molecular-based applications including PCR, array, etc.

TECHNICAL SPECIFICATIONS

Applicable For All sensitive downstream applications such as qPCR and Next-Generation Sequencing.
Elution Volume ≥ 150 µl
Equipment Centrifuge, Vacuum Source and Manifold, Microcentrifuge, Cell Disrupter/Pulverizer w/ 50 ml Tube Adapter
Processing Time ≤ 20 minutes
Processing Volume ≤ 500mg fungi or bacteria (wet weight), 5×109 bacterial cells, 5×108 yeast cells, or 5×107 mammalian cells
Purity Typical A260/A280 & A260/A230 ≥ 1.8
Sample Source Fungal and bacterial cell cultures, spores, pollen, nematodes, as well as other microorganisms can also be sampled.
Size Range Capable of recovering genomic DNA up to and above ≥40 kb. Typical fragment sizes range from 25 to 35 kb. If present, parasitic and viral DNA will also be recovered.
Type Total DNA
Yield ≤ 125 µg total DNA
Supplemental Info

Información adicional

Peso 0,0 kg
Dimensiones 0,0 × 0,0 × 0,0 cm