Description
DESCRIPTION
The Quick-DNA Plant/Seed Miniprep Kit is a plant DNA isolation kit designed for the simple, rapid isolation of inhibitor-free, PCR-quality DNA from a variety of plant sample sources including leaves, stems, buds, flowers, fruit, seeds, etc. The procedure is easy and can be completed in minutes: plant samples are rapidly and efficiently lysed by bead beating with our state of the art, ultra-high density BashingBeads. Polysaccharides, lipids, and polyphenols/tannins are removed from the DNA using our Zymo-Spin column technology. The eluted DNA is ideal for downstream molecular-based applications including PCR, arrays, etc.
Applicable For |
All sensitive downstream applications such as qPCR and Next-Generation Sequencing. |
Elution Volume |
≥ 50 µl |
Equipment |
Microcentrifuge, vortex, cell disruptor/pulverizer |
Processing Volume |
≤ 150 mg |
Purity |
A260/A280 nm ≥1.8. |
Sample Source |
Leaves, stems, buds, flowers, fruit, seeds, etc. |
Sample Storage |
DNA stored at ≤ -20°C. |
Size Range |
Capable of recovering genomic DNA up to and above 40 kb. Typical fragment sizes range from 25 kb – 35 kb. If present, parasitic and viral DNA will also be recovered. |
Type |
Total DNA |
Yield |
≤ 25 µg total DNA |
Supplemental Info |
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