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(D6010) Quick-DNA Fecal/Soil Microbe Miniprep Kit (50 prep)

HIGHLIGHTS

  • Rapid method for the isolation of inhibitor-free, PCR-quality DNA from fecal samples in minutes, including from humans, birds, rats, mice, cattle, etc.
  • Ultra-high density BashingBeads are fracture resistant and chemically inert.
  • Zymo-Spin column and unique filtration technologies effectively removes PCR inhibitors from the DNA product.
Protocol: 
Datasheet: 
SDS (MSDS): 
Optimized Lysis Protocols: 

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SKU DM0009DF Categorías ,
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Descripción

DESCRIPTION

The Quick-DNA Fecal/Soil Microbe Miniprep Kit is a fecal and soil DNA extraction kit designed for the simple and rapid isolation of inhibitor-free, PCR-quality host cell and microbial DNA from a variety of sample sources, including humans, birds, rats, mice, cattle, etc. The procedure is easy and can be completed in minutes: fecal samples are rapidly and efficiently lysed by bead beating with our state of the art, ultra-high density BashingBeads. Zymo-Spin column technology is then used to isolate the DNA, which is subsequently filtered to remove humic acids/polyphenols that can inhibit PCR. Eluted DNA is ideal for downstream molecular-based applications including PCR, arrays, genotyping, methylation detection, etc.

TECHNICAL SPECIFICATIONS

Applicable For All sensitive downstream applications such as qPCR and Next-Generation Sequencing.
Elution Volume ≥ 50 µl
Equipment Microcentrifuge, vortex, cell disruptor/pulverizer
Processing Volume ≤150 mg feces, ≤ 250 mg soil, ≤100 mg fungal/bacterial cells (wet weight)
Purity A260/A280 nm ≥1.8.
Sample Source Feces or soil
Sample Storage DNA stored at ≤ -20°C.
Size Range Capable of recovering genomic DNA up to and above 40 kb. In most instances, mitochondrial DNA and viral DNA (if present) will also be recovered.
Type Total DNA
Yield ≤ 25 µg total DNA
Supplemental Info

Información adicional

Peso 0,0 kg
Dimensiones 0,0 × 0,0 × 0,0 cm