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(D5005) EZ DNA Methylation-Gold™ Kit (50 Rxns)

HIGHLIGHTS
  • Complete bisulfite conversion of DNA in less than 3 hours.
  • Desulphonation and recovery of bisulfite-treated DNA with a spin column.
  • Recovered DNA is ideal for downstream analyses such as PCR, endonuclease digestion, sequencing, microarrays, etc.
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SKU DM0032EZ Categoría
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Descripción

DESCRIPTION

The EZ DNA Methylation-Gold Kit is a refinement of our popular EZ DNA Methylation kit and uses heat denaturation instead of chemical denaturation of the input DNA. This allows for the denaturation and bisulfite conversion steps to be consolidated into one step, leading to a much-reduced incubation time. Recovered bisulfite-converted DNA is ideal for PCR amplification for downstream analyses including endonuclease digestion, sequencing, microarrays, etc.

 

TECHNICAL SPECIFICATIONS
Applications Purified, converted DNA is of high-quality and well-suited for downstream processes, including library preparation for Next-generation sequencing, PCR amplification, etc.
Conversion >99%
Elution Volume ≥ 10 µl
Equipment Microcentrifuge and thermocycler with heated lid
Input 500 pg – 2 µg of DNA.
Processing Time 3 hours
Recovery >75%
Sample Source Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free.
Supplemental Info

Información adicional

Peso 0,0 kg
Dimensiones 0,0 × 0,0 × 0,0 cm

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