Descripción
DESCRIPTION
Part of the ZymoPURE Plasmid Kits collection, the ZymoPURE II Plasmid Midiprep kit provides the fastest and simplest method available to efficiently isolate up to 1.2 mg of transfection grade plasmid DNA from E. coli. Utilizing a modified alkaline lysis in conjunction with a patented binding system, the ZymoPURE II Midiprep kit can process up to 50 ml of culture in less than 18 minutes. This remarkable kit results in significantly more plasmid DNA while providing drastically reduced processing times. The plasmid DNA is rapidly bound onto a column with either a vacuum or a centrifuge instead of a slow gravity flow column. Additionally, there is no alcohol precipitation step required and the elution is performed using a microcentrifuge. The recovered plasmid DNA is highly concentrated (up to 6 µg/µl), endotoxin-free and transfection-ready. As an added convenience, the ZymoPURE II Plasmid Midiprep kit contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization.
Learn More
| Applicable For |
Transfection, transformation, lentivirus production, adenovirus production, AAV production, CRISPR, genome editing, in vivo studies, sequencing, restriction endonuclease digestion, in vitro transcription/translation, PCR, and other sensitive applications. |
| Binding Capacity |
1.2 mg |
| Culture Input |
≤ 50 ml |
| Elution Volume |
≥ 150 µl |
| Endotoxin Levels |
≤ 0.025 EU/µg of Plasmid DNA |
| Equipment |
Microcentrifuge and vacuum/vacuum manifold (recommended) or swinging bucket centrifuge |
| Format |
Spin-Column |
| Processing Time |
≤ 18 min |
| Purity |
Typical Abs 260/280 ≥1.8 and Abs 260/230≥ 2.0 |
| Size Range |
Up to 200 kb |
| Yield |
Up to 1.2 mg per preparation. Actual yield is dependent on the plasmid copy number, culture growth conditions, and strain of E. coli utilized. Typical yields from 50 ml of overnight culture grown in LB for high copy number plasmids are 200 – 400 µg. |
| Supplemental Info |
|
