Description
DESCRIPTION
The Oligo Clean & Concentrator provides a streamlined method for efficient recovery and clean-up of DNA/RNA fragments and oligonucletides ≥ 16 nt from labeling (radioactive, biotin, DIG, etc.) and other enzymatic reactions. Unincorporated nucleotides, short oligos, dyes, enzymes, and salts are effectively removed by the clean-up procedure. DNA/RNA is washed and concentrated into a small volume of water. Purified DNA/RNA, available in just 2 minutes, is suitable for hybridization, gel shift assays, enzymatic reactions, ligation, sequencing, microarray analysis, etc.
| Applicable For |
Single-stranded and double-stranded DNA and RNA |
| Elution Volume |
≥ 6 µl |
| Equipment |
Microcentrifuge |
| Purity |
A260/A280 > 1.8, A260/A230 > 1.8 |
| Sample Source |
Enzymatic reaction mixtures containing oligonucleotides ≥ 16 nt (radioactive-, biotin-, DIG-labeled, etc.) |
| Size Range |
For oligonucleotides ≥ 16 nt, up to 23 kb |
| Yield |
10 µg of ssDNA/RNA or 5 µg of dsDNA with a typical recovery > 90% |
