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(D2002) YeaStar™ Genomic DNA Kit (40 Preps) w/ Zymo-Spin™ III Columns (Uncapped)

HIGHLIGHTS

  • Simple: Fast spin-column procedure yields pure yeast genomic DNA without using glass beads or phenol.
  • Versatile: Efficient DNA isolation from a broad spectrum of fungal species susceptible to Zymolyase.
  • High-Quality: Isolated genomic DNA is ready for Southern blotting, PCR, restriction enzyme digestions, etc.
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SKU DM0032YD Categorías ,
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Descripción

DESCRIPTION

The YeaStar Genomic DNA Kit is designed for reliable and efficient isolation of genomic DNA from a broad spectrum of fungal species, including Aspergillus fumigatusAspergillus nidulansAspergillus nivens var. aureusCandida albicans, Pichia pastoris, Saccharomyces cerevisiaeSchizosaccharomyces pombe, and any fungi whose cell walls are susceptible to yeast lytic enzyme lysis. The kit is based on highly efficient enzyme lysis and Zymo-Spin column technology. Each standard prep yields about 7 – 20 µg of DNA with a size distribution of 35 – 60 kb. The resulting genomic DNA can be used for direct analysis including Southern blotting, PCR, restriction endonuclease digestion, etc.

TECHNICAL SPECIFICATIONS

Applicable For Isolated DNA is well suited for downstream applications such as Southern blotting, PCR, restriction endonuclease digestion, Next-Generation Sequencing and other sensitive applications.
Elution Volume ≥ 60 µl TE or DNase-free Water
Equipment Microcentrifuge, heat block/bath
Processing Time 1.5 hours
Processing Volume ≤ 1.5 ml of Culture (1-5 x 107 cells)
Purity Typical A260/A280 & A260/A230 ≥ 1.8
Sample Source Cell Culture (Colonies/Patches or Liquid Culture)
Size Range Capable of recovering 35-60 kb sized fragments of genomic and mitochondrial DNA.
Type Total DNA
Yield ≤ 25 µg of total DNA

Información adicional

Peso 0,0 kg
Dimensiones 0,0 × 0,0 × 0,0 cm